HERPES-SIMPLEX VIRUS-DNA ISOLATION FROM INFECTED-CELLS WITH A NOVEL PROCEDURE

被引:105
作者
PIGNATTI, PF
CASSAI, E
MENEGUZZI, G
CHENCINER, N
MILANESI, G
机构
[1] CNR,IST GENET,PAVIA,ITALY
[2] CNR,GENET BIOCHIM & EVOLUZ LAB,PAVIA,ITALY
[3] UNIV FERRARA,IST MICROBIOL,I-44100 FERRARA,ITALY
[4] FAC SCI NICE,CTR BIOCHIM,F-06034 NICE,FRANCE
关键词
D O I
10.1016/0042-6822(79)90295-2
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Herpes Simplex Virus type 1-infected cells were extracted in the presence of 0.25% Triton X-100-0.2 M NaCl. Viral DNA associated with proteins was found in the supernatant after low-speed centrifugation. Only viral DNA was recovered by this procedure, as shown by CsCl density analysis after deproteinization. Full-length viral DNA molecules were observed in the electron microscope. © 1979.
引用
收藏
页码:260 / 264
页数:5
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