ISOLATION OF AN HLA-A2.1 EXTRACTED HUMAN MINOR HISTOCOMPATIBILITY PEPTIDE

被引:19
作者
DEBUEGER, M
VERRECK, F
BLOKLAND, E
DRIJFHOUT, JW
AMONS, R
KONING, F
GOULMY, E
机构
[1] LEIDEN UNIV HOSP,DEPT IMMUNOHAEMATOL & BLOODBANK,RIJNSBURGERWEG 10,2333 AA LEIDEN,NETHERLANDS
[2] SILVIUS LABS,DEPT MED BIOCHEM,LEIDEN,NETHERLANDS
关键词
HUMAN CLASS-I; MINOR HISTOCOMPATIBILITY ANTIGEN;
D O I
10.1002/eji.1830230305
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Purified HLA-A2.1 molecules obtained by affinity chromatography of 6 x 10(10) Epstein Barr virus (EBV)-transformed B lymphocytes were used in an attempt to isolate the human HLA-A2.1-restricted minor histocompatibility (H) peptides H-Y and HA-2. Fraction 18 of the high-performance liquid chromatography (HPLC)-separated HLA-A2.1 peptide pool was found to contain the natural HA-2 peptide. An HA-2-specific, HLA-A2.1-restricted cytotoxic T lymphocyte clone lysed HLA-A2.1+ HA-2- EBV-transformed B lymphocyte cell lines reproducibly and in a concentration-dependent fashion in the presence of fraction 18, but not in the presence of other HPLC fractions. By contrast, H-Y sensitizing activity was not found in any fraction. Amino acid sequencing of peptide fraction 18 revealed a mixture of peptides with maximal length of nine amino acids, in which the presence of Leu at positions 2 and 9 was dominant. Surprisingly, the HA-2 peptide could not be mimicked by any of the peptide mixtures synthesized according to the amino acid sequences found in fraction 18. Our failure to obtain the actual amino acid sequence of the human minor H peptide HA-2 from a peptide pool with the established pattern for binding to HLA-A2.1 may indicate that this CTL defined minor H peptide does not represent an abundant HLA-A2.1 binding peptide.
引用
收藏
页码:614 / 618
页数:5
相关论文
共 21 条
  • [1] PEPTIDE BINDING TO CLASS-I MHC ON LIVING CELLS AND QUANTITATION OF COMPLEXES REQUIRED FOR CTL LYSIS
    CHRISTINCK, ER
    LUSCHER, MA
    BARBER, BH
    WILLIAMS, DB
    [J]. NATURE, 1991, 352 (6330) : 67 - 70
  • [2] T-IMMUNOGENIC PEPTIDES ARE CONSTITUTED OF RARE SEQUENCE PATTERNS - USE IN THE IDENTIFICATION OF T-EPITOPES IN THE HUMAN IMMUNODEFICIENCY VIRUS GAG PROTEIN
    CLAVERIE, JM
    KOURILSKY, P
    LANGLADEDEMOYEN, P
    CHALUFOURPROCHNICKA, A
    DADAGLIO, G
    TEKAIA, F
    PLATA, F
    BOUGUELERET, L
    [J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 1988, 18 (10) : 1547 - 1553
  • [3] ENDOGENOUS RETROVIRUSES LEAD TO THE EXPRESSION OF A HISTOCOMPATIBILITY ANTIGEN DETECTABLE BY SKIN-GRAFT REJECTION
    COLOMBO, MP
    JAENISCH, R
    WETTSTEIN, PJ
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (01) : 189 - 193
  • [4] DEBRUEGER M, 1992, J IMMUNOL, V149, P1788
  • [5] CELLULAR PEPTIDE COMPOSITION GOVERNED BY MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I MOLECULES
    FALK, K
    ROTZSCHKE, O
    RAMMENSEE, HG
    [J]. NATURE, 1990, 348 (6298) : 248 - 251
  • [6] ALLELE-SPECIFIC MOTIFS REVEALED BY SEQUENCING OF SELF-PEPTIDES ELUTED FROM MHC MOLECULES
    FALK, K
    ROTZSCHKE, O
    STEVANOVIC, S
    JUNG, G
    RAMMENSEE, HG
    [J]. NATURE, 1991, 351 (6324) : 290 - 296
  • [7] Y-ANTIGEN KILLING BY T-CELLS OF WOMEN IS RESTRICTED BY HLA
    GOULMY, E
    TERMIJTELEN, A
    BRADLEY, BA
    VANROOD, JJ
    [J]. NATURE, 1977, 266 (5602) : 544 - 545
  • [8] GOULMY E, 1988, TRANSPLANT REV, V2, P29, DOI DOI 10.1016/S0955-470X(88)80005-3
  • [9] HUTN DF, 1992, SCIENCE, V255, P1261
  • [10] JARDETZKY TS, 1991, ANTURE, V353, P326