SPECIFIC REGIONS OF ESCHERICHIA-COLI OMPF PROTEIN INVOLVED IN ANTIGENIC AND COLICIN RECEPTOR-SITES AND IN STABLE TRIMERIZATION

被引：42
作者：
FOUREL, D
MIZUSHIMA, S
BERNADAC, A
PAGES, JM
机构：
[1] CNRS,UPR 9027,GDR 1000,31 CHEMIN JOSEPH AIGUIER,BP 71,F-13402 MARSEILLE 20,FRANCE
[2] UNIV TOKYO,INST APPL MICROBIOL,BUNKYO KU,TOKYO 113,JAPAN
来源：
JOURNAL OF BACTERIOLOGY | 1993年 / 175卷 / 09期
关键词：
D O I：
10.1128/JB.175.9.2754-2757.1993
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Four different mutations were obtained by selecting for resistance to colicin N and screening for continued production of the OmpF protein of Escherichia coli. Two of them also conferred resistance to colicin A. The substitutions C for R-168 (R168C) and E284K caused the loss of the E21 epitope, while the transition G285D altered the E18, E19, and E20 antigenic sites. The substitution G119D drastically affected the stability of the trimeric conformation.
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页码：2754 / 2757
页数：4
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