CHARACTERIZATION OF AXONALLY TRANSPORTED [I-125] PYY BINDING-SITES IN RAT VAGUS NERVE

被引：12

作者：

CORP, ES ^{[1
]}

SMITH, GP ^{[1
]}

机构：

[1] CORNELL UNIV,MED CTR,COLL MED,DEPT PSYCHIAT,NEW YORK,NY 10021

来源：

BRAIN RESEARCH | 1991年 / 553卷 / 01期

关键词：

NEUROPEPTIDE-Y; PARASYMPATHETIC NERVOUS SYSTEM; RECEPTOR AUTORADIOGRAPHY; Y2; RECEPTOR; PEPTIDE YY13-36; PRO34]NPY;

D O I：

10.1016/0006-8993(91)90248-T

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Quantitative receptor autoradiography was used to characterize [I-125]PYY binding to slide-mounted sections of rat cervical vagus nerve which had been ligated 24 h prior to extraction. Saturation and competitive binding characteristics were determined for the accumulation of binding sites detected in the segment of the nerve proximal to the ligature. High and low affinity binding components were resolved (K(d) = 93 pM and 2.1 nM). The rank order of potency for competing ligands was PYY > NPY >> PYY13-36 >>> [Pro34]NPY suggesting that vagal PYY/NPY binding sites are PYY-preferring and of the Y2 type.

引用

页码：175 / 179

页数：5

共 35 条

[1] HUMAN DISTRIBUTION AND RELEASE OF A PUTATIVE NEW GUT HORMONE, PEPTIDE-YY [J].

ADRIAN, TE ;

FERRI, GL ;

BACARESEHAMILTON, AJ ;

FUESSL, HS ;

POLAK, JM ;

BLOOM, SR .

GASTROENTEROLOGY, 1985, 89 (05) :1070-1077

[2] NEUROPEPTIDE-Y DISTRIBUTION IN HUMAN-BRAIN [J].

ADRIAN, TE ;

ALLEN, JM ;

BLOOM, SR ;

GHATEI, MA ;

ROSSOR, MN ;

ROBERTS, GW ;

CROW, TJ ;

TATEMOTO, K ;

POLAK, JM .

NATURE, 1983, 306 (5943) :584-586

[3] NEUROPEPTIDE-Y (NPY) BINDING-SITES IN RAT-BRAIN LABELED WITH I-125 BOLTON-HUNTER NPY - COMPARATIVE POTENCIES OF VARIOUS POLYPEPTIDES ON BRAIN NPY BINDING AND BIOLOGICAL RESPONSES IN THE RAT VASDEFERENS [J].

CHANG, RSL ;

LOTTI, VJ ;

CHEN, TB ;

CERINO, DJ ;

KLING, PJ .

LIFE SCIENCES, 1985, 37 (22) :2111-2122

[4] THE ANATOMY OF NEUROPEPTIDE-Y-CONTAINING NEURONS IN RAT-BRAIN [J].

CHRONWALL, BM ;

DIMAGGIO, DA ;

MASSARI, VJ ;

PICKEL, VM ;

RUGGIERO, DA ;

ODONOHUE, TL .

NEUROSCIENCE, 1985, 15 (04) :1159-1181

[5] NEUROPEPTIDE Y (NPY)-INDUCED FEEDING-BEHAVIOR IN FEMALE RATS - COMPARISON WITH HUMAN NPY ([MET17]NPY), NPY ANALOG ([NORLEU4]NPY) AND PEPTIDE-YY [J].

CLARK, JT ;

SAHU, A ;

KALRA, PS ;

BALASUBRAMANIAM, A ;

KALRA, SP .

REGULATORY PEPTIDES, 1987, 17 (01) :31-39

[6] EFFECT OF 4TH VENTRICULAR NEUROPEPTIDE-Y AND PEPTIDE-YY ON INGESTIVE AND OTHER BEHAVIORS [J].

CORP, ES ;

MELVILLE, LD ;

GREENBERG, D ;

GIBBS, J ;

SMITH, GP .

AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (02) :R317-R323

[7] PEPTIDE YY-LIKE IMMUNOREACTIVITY IN THE CENTRAL-NERVOUS-SYSTEM OF THE RAT [J].

EKMAN, R ;

WAHLESTEDT, C ;

BOTTCHER, G ;

SUNDLER, F ;

HAKANSON, R ;

PANULA, P .

REGULATORY PEPTIDES, 1986, 16 (02) :157-168

[8] THE DISTRIBUTION AND ONTOGENY OF POLYPEPTIDE YY (PYY)-IMMUNOREACTIVE AND PANCREATIC-POLYPEPTIDE (PP)-IMMUNOREACTIVE CELLS IN THE GASTROINTESTINAL-TRACT OF RAT [J].

ELSALHY, M ;

WILANDER, E ;

JUNTTIBERGGREN, L ;

GRIMELIUS, L .

HISTOCHEMISTRY, 1983, 78 (01) :53-60

[9] IMMUNOCYTOCHEMICAL IDENTIFICATION OF POLYPEPTIDE-YY (PYY) CELLS IN THE HUMAN GASTROINTESTINAL-TRACT [J].

ELSALHY, M ;

GRIMELIUS, L ;

WILANDER, E ;

RYBERG, B ;

TERENIUS, L ;

LUNDBERG, JM ;

TATEMOTO, K .

HISTOCHEMISTRY, 1983, 77 (01) :15-23

[10] [LEU31,PRO34]NEUROPEPTIDE-Y - A SPECIFIC Y-1 RECEPTOR AGONIST [J].

FUHLENDORFF, J ;

GETHER, U ;

AAKERLUND, L ;

LANGELANDJOHANSEN, N ;

THOGERSEN, H ;

MELBERG, SG ;

OLSEN, UB ;

THASTRUP, O ;

SCHWARTZ, TW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (01) :182-186

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