A COMPARATIVE-ASSESSMENT OF PURIFICATION TECHNIQUES FOR MESOPHYLL PROTOPLASTS OF BRASSICA-NAPUS L

被引：6

作者：

MILLAM, S ^{[1
]}

BURNS, ATH ^{[1
]}

HOCKING, TJ ^{[1
]}

机构：

[1] POLYTECH WOLVERHAMPTON,SCH APPL SCI,WOLVERHAMPTON WV1 1SB,W MIDLANDS,ENGLAND

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1991年 / 24卷 / 01期

关键词：

BRASSICA-NAPUS; PROTOPLASTS; PURIFICATION;

D O I：

10.1007/BF00044264

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effects of three different general purification protocols have been assessed quantitatively using mesophyll protoplasts of Brassica napus. Within the initial sample two distinct sub-populations were determined. The methods used influenced the ratio of the vacuolated to chloroplastic type protoplast sub-populations. Overall recovery rates of the initial sample varied according to the method used from 38% to 27%, but the relative recovery of the sub-populations varied considerably with a purified ration of between 1.0:0.78 to 1.0:7.0. Size distribution profiles of the initial and purified populations are also presented.

引用

页码：43 / 48

页数：6

共 22 条

[1]

ALEXANDER R, 1983, 6TH INT PROT S BAS, P62

[2] A RAPID AND EFFICIENT ALTERNATIVE PROCEDURE FOR THE REGENERATION OF PLANTS FROM HYPOCOTYL PROTOPLASTS OF BRASSICA-NAPUS [J].

BARSBY, TL ;

YARROW, SA ;

SHEPARD, JF .

PLANT CELL REPORTS, 1986, 5 (02) :101-103

[3]

CONSTABEL F, 1985, PLANT PROTOPLASTS, P54

[4] ASPECTS OF PROTOPLAST CULTURE AND PLANT-REGENERATION [J].

DAVEY, MR ;

POWER, JB .

PLANT CELL TISSUE AND ORGAN CULTURE, 1988, 12 (02) :115-125

[5]

DAVEY MR, 1983, 6TH P INT PROT S E S, V46, P2

[6] ISOLATION AND PROPERTIES OF FUNCTIONAL MESOPHYLL PROTOPLASTS AND CHLOROPLASTS FROM ZEA-MAYS [J].

DAY, DA ;

JENKINS, CLD ;

HATCH, MD .

AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1981, 8 (01) :21-29

[7]

Dodds J.H., 1985, EXPT PLANT TISSUE CU

[8]

GAMBORG OL, 1981, PROTOPLASMA, V106, P351

[9] HIGH GROWTH-RATE AND REGENERATION CAPACITY OF HYPOCOTYL PROTOPLASTS IN SOME BRASSICACEAE [J].

GLIMELIUS, K .

PHYSIOLOGIA PLANTARUM, 1984, 61 (01) :38-44

[10] ISOLATION, CULTURE, AND INDUCTION OF EMBRYOGENESIS IN PROTOPLASTS FROM CELL-SUSPENSIONS OF ATROPA-BELLADONNA [J].

GOSCH, G ;

BAJAJ, YPS ;

REINERT, J .

PROTOPLASMA, 1975, 86 (04) :405-410

← 1 2 3 →