APPLICATION OF THE UGI REACTION FOR THE PREPARATION OF ENZYME ELECTRODES

Glucose oxidase and catalase were immobilized via the Ugi reaction by means of cyclohexyl isocyanide and glutaraldehyde on a nylon net partially hydrolysed by hydrochloric acid. A specific enzyme sensor for D-glucose was made by fixing the nylon net with immobilized enzymes on the tip of a Clark-type oxygen sensor. For comparison purposes glucose oxidase and catalase were also co-immobilized in the absence of cyclohexyl isocyanide or only glucose oxidase was immobilized with and without cyclohexyl isocyanide. The prepared biosensors were characterized by the specific activity of glucose oxidase and its dependence on Ph and temperature and by the apparent Michaelis constant. The linear range of the biosensor response to the substrate concentration and the stability of the biosensor were determined. The long-term stabilities of the enzyme electrodes were compared and the advangtage of the developed method was demonstrated. © 1990.