THE OPTIMIZATION OF IMMUNOGLOBULIN SECRETION INVITRO BY MOUSE SPLEEN-CELLS AND HYBRIDOMA CELLS

Optimal culture conditions and cell densities for antibody production by spleen cells from mice infected with Trypanosoma brucei, and by hybridoma cells, were assessed in vitro by a silver-immunogold (SIG) blot technique and an enzyme-linked immunosorbent assay (dELISA). The blot assay measured the proportion of secreting cells in the population and the ELISA measured the quantity of synthesised and secreted immunoglobulin (Ig) in cells lysates and 3-h culture supernatants. Marked inhibition of Ig secretion occured when cells were maintain in rotating cultures, no inhibition was seen. These cells maintain a steady rate of Ig synthesis and secretion over 3 h. © 1990.