HEPATIC LIPID-METABOLISM - EFFECT OF SPERMINE, ALBUMIN, AND Z-PROTEIN ON MICROSOMAL LIPID FORMATION

被引：50

作者：

JAMDAR, SC

机构：

[1] MED COLL VIRGINIA,DEPT INTERNAL MED,RICHMOND,VA 23298

[2] MED COLL VIRGINIA,DEPT BIOCHEM,RICHMOND,VA 23298

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1979年 / 195卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-9861(79)90329-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Effects of spermine, bovine serum albumin, and Z protein on microsomal lipid formation from sn-glycerol 3-phosphate and [14C]palmitoyl CoA were investigated. In the presence of these agents, microsomal lipid formation was stimulated. This was attributed to the activation of sn-glycerol 3-phosphate acyltransferase and to the inhibition of palmitoyl CoA hydrolase. In addition to palmitoyl CoA, spermine also reacted with microsomal membranes in causing their aggregation, and ATP reversed the effect of spermine. Further studies indicated that the interaction of spermine with palmitoyl CoA, rather than with microsomal membranes, was responsible for the activation of glycerolipid formation or to the inhibition of palmitoyl CoA reductase. Examination of the intravesicular distribution of sn-glycerol 3-phosphate acyltransferase and palmitoyl CoA hydrolase and the effects of structural integrity of microsomal vesicles on these two membrane-bound enzymes indicated that the activation of glycerolipid formation and the inhibition of palmitoyl CoA hydrolase by spermine, bovine serum albumin, or Z protein may be closely linked with the structural integrity of microsomal vesicles. © 1979.

引用

页码：81 / 94

页数：14

共 27 条

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[12]

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