NEW SUPERCOMPLEXES OF THE OXIDATIVE PHOSPHORYLATION SYSTEM IN PEA (Pisum sativum L.) SEEDLINGS MITOCHONDRIA

被引：1

作者：

Kondakova, M. A. ^{[1
]}

Ukolova, I. V. ^{[1
]}

Borovskii, G. B. ^{[1
]}

Voinikov, V. K. ^{[1
]}

机构：

[1] Siberian Inst Plant Physiol & Biochem SB RAS, 132 Lermontov St, Irkutsk 664033, Russia

来源：

IZVESTIYA VUZOV-PRIKLADNAYA KHIMIYA I BIOTEKHNOLOGIYA | 2016年 / 6卷 / 03期

关键词：

supercomplexes; mitochondria; oxidative phosphorylation system; Pisum sativum L;

D O I：

10.21285/2227-2925-2016-6-3-143-146

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The organization of the oxidative phosphorylation system (OXPHOS) in mitochondria isolated from etiolated pea (Pisum sativum L.) seedlings was studied by 1D BN-PAGE, 2D BNE/BNE u 2D BNE/SDS with following histochemical staining, immunochemical and MALDI TOF mass spectrometry analysis and as a result new supercomplexes were revealed. It was shown that pea mitochondria OXPHOS comprises the respirasome I+III2+IVn being earlier detected in other plant species and new supercomplexes, such as a major respirasome I+NDA+III2+IV, supercomplex NDA+NDB+III2+IV, megacomplex (II+III2+IV)(n), and ATP-synthasome - NDA+NDB+AOX+ATP-synthase. The association of the alternative respiratory enzymes with other OXPHOS complexes could improve adaptive potential of plant mitochondria.

引用

页码：143 / 146

页数：4

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