CHARACTERIZATION AND APPLICATION OF GLUCOSE-OXIDASE IMMOBILIZED IN SILICA HYDROGEL

被引：0

作者：

KOJUHAROVA, A ^{[1
]}

POPOVA, Y ^{[1
]}

KIROVA, N ^{[1
]}

KLISSURSKI, D ^{[1
]}

SIMEONOV, D ^{[1
]}

SPASOV, L ^{[1
]}

机构：

[1] BULGARIAN ACAD SCI, INST GEN & INORGAN CHEM, BU-1040 SOFIA, BULGARIA

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 1988年 / 42卷 / 02期

关键词：

CHEMICAL OPERATIONS - High Temperature Effects - CHEMICAL REACTIONS - pH Effects - SILICA GEL - SUGAR - Oxidation;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The properties of glucose oxidase (EC1134) embedded in silica gel were investigated. The immobilized preparations retained 62 percent of the activity of the mobilized enzyme. The optimum pH value (5. 8) and temperature (30 degree C) were the same for the immobilized as for the soluble enzyme, but immobilized glucose oxidase had higher thermal and pH-stability. The constants of thermal and pH inactivation, and the Michaelis constants were calculated. The effects of various cations and proteins on the activity of the immobilized enzyme were studied; NH//4, K**P**L**U, Mg** plus and gelatin all increased the relative activity of the preparations to 93-96 percent of the soluble enzyme. Combined immobilized preparations of glucose oxidase and catalase were repeatedly used in the oxidation of D-glucose and the preparation of calcium gluconate.

引用

页码：95 / 104

页数：10

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