HIGH-RESOLUTION VISUAL MAPPING OF STRETCHED DNA BY FLUORESCENT HYBRIDIZATION

被引：244

作者：

PARRA, I ^{[1
]}

WINDLE, B ^{[1
]}

机构：

[1] CANC THERAPY & RES CTR S TEXAS,8122 DATAPOINT DR,SAN ANTONIO,TX 78229

来源：

NATURE GENETICS | 1993年 / 5卷 / 01期

关键词：

D O I：

10.1038/ng0993-17

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi-colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a >200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.

引用

页码：17 / 21

页数：5

共 17 条

[1] AMPLIFIED N-MYC IN HUMAN NEURO-BLASTOMA CELLS IS OFTEN ARRANGED AS CLUSTERED TANDEM REPEATS OF DIFFERENTLY RECOMBINED DNA [J].

AMLER, LC ;

SCHWAB, M .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (11) :4903-4913

[2]

BALLANNECHANTEL.B, 1992, CELL, V70, P1059

[3] RADIATION HYBRID MAPPING - A SOMATIC-CELL GENETIC METHOD FOR CONSTRUCTING HIGH-RESOLUTION MAPS OF MAMMALIAN CHROMOSOMES [J].

COX, DR ;

BURMEISTER, M ;

PRICE, ER ;

KIM, S ;

MYERS, RM .

SCIENCE, 1990, 250 (4978) :245-250

[4]

EVANS GA, 1987, METHOD ENZYMOL, V152, P604

[5] CHROMOSOMAL REGION OF THE CYSTIC-FIBROSIS GENE IN YEAST ARTIFICIAL CHROMOSOMES - A MODEL FOR HUMAN GENOME MAPPING [J].

GREEN, ED ;

OLSON, MV .

SCIENCE, 1990, 250 (4977) :94-98

[6] HIGH-RESOLUTION MAPPING OF MAMMALIAN GENES BY INSITU HYBRIDIZATION TO FREE CHROMATIN [J].

HENG, HHQ ;

SQUIRE, J ;

TSUI, LC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (20) :9509-9513

[7] EXTENDING THE CAPABILITIES OF INTERPHASE CHROMATIN MAPPING [J].

LAWRENCE, JB ;

CARTER, KC ;

GERDES, MJ .

NATURE GENETICS, 1992, 2 (03) :171-172

[8] INTERPHASE AND METAPHASE RESOLUTION OF DIFFERENT DISTANCES WITHIN THE HUMAN DYSTROPHIN GENE [J].

LAWRENCE, JB ;

SINGER, RH ;

MCNEIL, JA .

SCIENCE, 1990, 249 (4971) :928-932

[9] HIGH-RESOLUTION MAPPING OF HUMAN CHROMOSOME-11 BY INSITU HYBRIDIZATION WITH COSMID CLONES [J].

LICHTER, P ;

TANG, CJC ;

CALL, K ;

HERMANSON, G ;

EVANS, GA ;

HOUSMAN, D ;

WARD, DC .

SCIENCE, 1990, 247 (4938) :64-69

[10] ORGANIZATION AND GENESIS OF DIHYDROFOLATE-REDUCTASE AMPLICONS IN THE GENOME OF A METHOTREXATE-RESISTANT CHINESE-HAMSTER OVARY CELL-LINE [J].

MA, C ;

LOONEY, JE ;

LEU, TH ;

HAMLIN, JL .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (06) :2316-2327

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