PHYSICAL MAPPING OF THE LOCI GABRA3, DXPAS8, CAML1, AND RSVP IN A REGION OF THE MOUSE X-CHROMOSOME HOMOLOGOUS TO HUMAN XQ28

被引：27
作者：
FAUST, CJ
HERMAN, GE
机构：
[1] BAYLOR COLL MED,INST MOLEC GENET,ONE BAYLOR PLAZA,S911,HOUSTON,TX 77030
[2] BAYLOR COLL MED,DEPT PEDIAT,HOUSTON,TX 77030
来源：
GENOMICS | 1991年 / 11卷 / 01期
关键词：
D O I：
10.1016/0888-7543(91)90112-R
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Using pulsed-field gel electrophoresis, a 3 million-bp physical map containing the X-linked loci Gabra3, DXPas8, CamL1, and Rsvp has been constructed for a segment of the mouse X chromosome homologous to human Xq28. Detailed mapping was performed using single and double digestions with rare-cutter restriction enzymes. Gabra3 and DXPas8 have been shown to be physically linked within a maximal distance of 1600 kb, DXPas8 and CamL1 within 750 kb, and CamL1 and Rsvp within 450 kb. In addition, several CpG islands have been detected in the region encompassing CamL1 and Rsvp. These studies confirm a gene order of cen-Gabra3-DXPas8-CamL1-Rsvp-tel determined by genetic mapping in interspecific backcrosses (A. S. Ryder-Cook et al. 1988, EMBO J. 7: 3017-3021; G. E. Herman et al. 1991, Genomics 9: 670-677). Physical distances for the loci studied agree with the calculated genetic distances. Assuming that there is conserved linkage between man and mouse in the region, the physical mapping data presented here may help to clarify the uncertain gene order for some human Xq28 loci. © 1991.
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页码：154 / 164
页数：11
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