TUNICAMYCIN-MEDIATED DEPLETION OF INSULIN RECEPTORS IN 3T3-L1 ADIPOCYTES

被引：109
作者：
ROSEN, OM
CHIA, GH
FUNG, C
RUBIN, CS
机构：
[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MED,BRONX,NY 10461
[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT NEUROSCI,BRONX,NY 10461
来源：
JOURNAL OF CELLULAR PHYSIOLOGY | 1979年 / 99卷 / 01期
关键词：
D O I：
10.1002/jcp.1040990106
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Tunicamycin, an antibiotic that inhibits protein glycosylation, elicited a rapid depletion of insulin binding activity at the surface of 3T3‐L1 adipocytes. Disappearance of insulin receptors occurred more rapidly in the presence of tunicamycin than when protein synthesis was inhibited by cycloheximide and was accompanied by a diminution in sensitivity of the adipocytes to the acute effects of insulin and anit‐insulin receptor antibody on hexose uptake and metabolism. Copyright © 1979 Wiley‐Liss, Inc.
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页码：37 / 42
页数：6
相关论文
共 34 条
[1]  
CHEN WW, 1977, J BIOL CHEM, V252, P3473
[2]   AFFINITY CHROMATOGRAPHY AND PURIFICATION OF INSULIN RECEPTOR OF LIVER-CELL MEMBRANES [J].
CUATRECASAS, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1972, 69 (05) :1277-+
[3]  
DUKSIN D, 1977, J BIOL CHEM, V252, P955
[4]   CHANGES IN SURFACE PROPERTIES OF NORMAL AND TRANSFORMED-CELLS CAUSED BY TUNICAMYCIN, AN INHIBITOR OF PROTEIN GLYCOSYLATION [J].
DUKSIN, D ;
BORNSTEIN, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3433-3437
[5]   INSULIN-INDUCED DISSOCIATION OF ITS RECEPTOR INTO SUBUNITS - POSSIBLE MOLECULAR CONCOMITANT OF NEGATIVE COOPERATIVITY [J].
GINSBERG, BH ;
KAHN, CR ;
ROTH, J ;
DEMEYTS, P .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1976, 73 (04) :1068-1074
[6]   SPONTANEOUS HERITABLE CHANGES LEADING TO INCREASED ADIPOSE CONVERSION IN 3T3-CELLS [J].
GREEN, H ;
KEHINDE, O .
CELL, 1976, 7 (01) :105-113
[7]   ESTABLISHED PRE-ADIPOSE CELL LINE AND ITS DIFFERENTIATION IN CULTURE [J].
GREEN, H ;
MEUTH, M .
CELL, 1974, 3 (02) :127-133
[8]   SUBLINES OF MOUSE 3T3 CELLS THAT ACCUMULATE LIPID [J].
GREEN, H ;
KEHINDE, O .
CELL, 1974, 1 (03) :113-116
[9]   ESTABLISHED PRE-ADIPOSE CELL LINE AND ITS DIFFERENTIATION IN CULTURE .2. FACTORS AFFECTING ADIPOSE CONVERSION [J].
GREEN, H ;
KEHINDE, O .
CELL, 1975, 5 (01) :19-27
[10]  
HICKMAN S, 1977, J BIOL CHEM, V252, P4402
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