ENZYMATIC DIGESTION OF DNA GAMMA-IRRADIATED IN AQUEOUS-SOLUTION SEPARATION OF DIGESTS BY ION-EXCHANGE CHROMATOGRAPHY

被引：23

作者：

DIZDAROGLU, M

HERMES, W

SCHULTEFROHLINDE, D

VONSONNTAG, C

机构：

[1] Institut für Strahlenchemie im Max-Planck-Institut für Kohlenforschung, D-4330, Mülheim a.d. Ruhr

来源：

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1978年 / 33卷 / 06期

关键词：

D O I：

10.1080/09553007814550471

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aqueous solutions of DNA were γirradiated in the presence and absence of oxygen and enzymatically hydrolysed by the combined action of pancreatic deoxyribonuclease (DNase I), snake-venom phosphodiesterase (PDE I), spleen phosphodiesterase (PDE II) and alkaline phosphatase. In contrast to unirradiated DNA, which is fully hydrolysed to nucleosides by these enzymes, γirradiated DNA yields a series of oligonucleotides. Their isolation might enable the future identification of the chemical nature of DNA lesions. © 1978 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：563 / 569

页数：7

共 27 条

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[9] STRAND BREAKS AND SUGAR RELEASE BY GAMMA-IRRADIATION OF DNA IN AQUEOUS-SOLUTION [J].

DIZDAROGLU, M ;

SONNTAG, CV ;

SCHULTEFROHLINDE, D .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1975, 97 (08) :2277-2278

[10] ISOLATION OF 2-DEOXY-D-ERYTHRO-PENTONIC ACID FROM AN ALKALI-LABILE SITE IN GAMMA-IRRADIATED DNA [J].

DIZDAROGLU, M ;

SCHULTEFROHLINDE, D ;

VONSONNTAG, C .

INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1977, 32 (05) :481-483

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