A SYNTHETIC PEPTIDE HOMOLOGOUS TO RETROVIRAL ENVELOPE PROTEIN DOWN-REGULATES TNF-ALPHA AND IFN-GAMMA MESSENGER-RNA EXPRESSION

被引：16

作者：

HARAGUCHI, S

GOOD, RA

CIANCIOLO, GJ

DAY, NK

机构：

[1] UNIV S FLORIDA,ALL CHILDRENS HOSP,DEPT PEDIAT,801 6TH ST S,ST PETERSBURG,FL 33701

[2] MACRONEX INC,MORRISVILLE,NC

来源：

JOURNAL OF LEUKOCYTE BIOLOGY | 1992年 / 52卷 / 04期

关键词：

RETROVIRAL ENVELOPE PROTEIN; IMMUNOSUPPRESSIVE SYNTHETIC PEPTIDE; CKS-17; CYTOKINE GENE EXPRESSION; TNF-ALPHA; IFN-GAMMA;

D O I：

10.1002/jlb.52.4.469

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We investigated the influence of CKS-17, a synthetic heptadecapeptide that corresponds to a highly conserved domain of the immunosuppressive retroviral envelope protein pl5E, on staphylococcal enterotoxin B (SEB)-induced TNF-alpha gene expression in human peripheral blood mononuclear cells and highly purified human monocyte preparations, as well as the production of TNF-alpha protein, using human peripheral blood mononuclear cells. RNA hybridization studies show that CKS-17 inhibits SEB-induced TNF-alpha mRNA accumulation in human peripheral blood mononuclear cells and human monocytes. CKS-17 is also shown to be highly suppressive for SEB-induced production of TNF-alpha proteins. Similarly, CKS-17 inhibits expression of SEB-induced IFN-gamma mRNA in human peripheral blood mononuclear cells. These results suggest that CKS-17 down-regulates both TNF-alpha and IFN-gamma production at mRNA level.

引用

页码：469 / 472

页数：4

共 23 条

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