PURIFICATION AND SOME PROPERTIES OF CANDIDA-ALBICANS DNA-POLYMERASES

被引：1

作者：

JAKAB, E ^{[1
]}

SCHMIDT, T ^{[1
]}

HERNADI, F ^{[1
]}

机构：

[1] DEBRECEN UNIV MED,SCH MED,INST PHARMACOL,DEPT CHEMOTHERAPY,H-4012 DEBRECEN,HUNGARY

来源：

PREPARATIVE BIOCHEMISTRY | 1991年 / 21卷 / 2-3期

基金：

匈牙利科学研究基金会;

关键词：

D O I：

10.1080/10826069108018007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA polymerases of Candida albicans were purified to near homogenity. Three well distinguished peaks of DNA polymerase activity (Enzyme I, II and III respectively) were obtained by DEAE-Sephacel chromatography. This purification step was followed by column chromatographies on Sepharose 6B and denatured DNA-cellulose. The enzymes' molecular mass and biochemical properties, including their inhibition by aphidicolin, were studied. Molecular mass was determined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and was found to be 110 kDa for Enzyme I, 80 kDa for Enzyme II and 50 kDa for Enzyme III.

引用

页码：105 / 123

页数：19

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