CLONING AND SEQUENCING OF THE GENE CODING FOR DEXTRANASE FROM STREPTOCOCCUS-SALIVARIUS

被引：34

作者：

OHNISHI, Y

KUBO, S

ONO, Y

NOZAKI, M

GONDA, Y

OKANO, H

MATSUYA, T

MATSUSHIRO, A

MORITA, T

机构：

[1] OSAKA UNIV,MICROBIAL DIS RES INST,DEPT MICROBIAL GENET,SUITA,OSAKA 565,JAPAN

[2] OSAKA UNIV,FAC DENT,DEPT ORAL & MAXILLOFACIAL SURG 1,SUITA,OSAKA 565,JAPAN

[3] OSAKA DENT UNIV,DEPT ORAL & MAXILLOFACIAL SURG 2,CHUOH KU,OSAKA,JAPAN

[4] OSAKA DENT UNIV,DEPT PROSTHODONT 1,CHUOH KU,OSAKA,JAPAN

来源：

GENE | 1995年 / 156卷 / 01期

关键词：

RECOMBINANT DNA; SIGNAL PEPTIDE; ORAL BACTERIUM; DENTAL CARIES;

D O I：

10.1016/0378-1119(95)00071-D

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We cloned and sequenced the dextranase (Dex) (1,6-alpha-glucanhydrolase; EC 3.2.1.11)-encoding gene from Streptococcus salivarius (Ss) strain M-33. Recombinant clones from an Ss genomic library specifying Dex activity were identified as colonies surrounded by transparent halos on blue dextran plates. One of the clones had a 4.3-kb KpnI fragment containing the gene coding for an 826-amino-acid polypeptide with a molecular mass of 87.9 kDa, which corresponds well to that of native Dex from the Ss culture supernatant. There was no sequence homology between the gene encoding Ss Dex and the gene encoding dextran glucosidase of S. mutans, or between their protein products.

引用

页码：93 / 96

页数：4

共 18 条

[1] PURIFICATION AND PARTIAL CHARACTERIZATION OF THE MULTICOMPONENT DEXTRANASE COMPLEX OF STREPTOCOCCUS-SOBRINUS AND CLONING OF THE DEXTRANASE GENE [J].