EFFECT OF SILVER-NITRATE AND 2,4-D ON ANTHER CULTURE OF BRUSSELS-SPROUTS (BRASSICA-OLERACEA VAR GEMMIFERA)

The response to anther culture, of six genotypes of Brussels sprouts was tested on six media which included two levels of 2,4-D and the presence or absence of silver nitrate. The presence of silver was usually beneficial, and with some genotypes had a very large effect. Increasing 2,4-D could be beneficial in the absence of silver nitrate, but was sometimes detrimental in the presence of silver. Replacing agar with highly purified agarose was not particularly beneficial. Genotype, medium and genotype X medium interactions were all significant factors, with genotype being the most important.