COMPARATIVE INVESTIGATIONS OF THE GENOTOXIC EFFECTS OF METALS IN THE SINGLE-CELL GEL (SCG) ASSAY AND THE SISTER-CHROMATID EXCHANGE (SCE) TEST

被引：126
作者：
HARTMANN, A [1 ]
SPEIT, G [1 ]
机构：
[1] UNIV ULM,KLIN GENET ABT,D-89069 ULM,GERMANY
来源：
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS | 1994年 / 23卷 / 04期
关键词：
CADMIUM; ARSENIC; HUMAN LYMPHOCYTES; IN-VITRO TEST;
D O I：
10.1002/em.2850230407
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
Sodium arsenite (NaAsO2) and cadmium sulphate (CdSO4) were tested for their ability to induce genotoxic effects in the single cell gel (SCG) assay and the sister chromatid exchange (SCE) test in human blood cultures in vitro. Both metals induced DNA damage in white blood cells that was expressed and detected as DNA migration in the SCG assay. Dose dependent effects were seen for cadmium in concentrations from 5 x 10(-4)-5 x 10(-3) M and for arsenic in concentrations from 2 x 10(-4)-1.5 x 10(-3) M. The distribution of DNA migration among cells, a function of dose, revealed that the majority of exposed cells expressed more DNA damage then cells from control cultures and that with increasing length of DNA migration the variability in migration among cells increased as well.
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收藏
页码：299 / 305
页数：7
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