DOMAINS AND THE HINGE REGION OF AN IMMUNOGLOBULIN HEAVY-CHAIN ARE ENCODED IN SEPARATE DNA SEGMENTS

被引：282
作者：
SAKANO, H
ROGERS, JH
HUPPI, K
BRACK, C
TRAUNECKER, A
MAKI, R
WALL, R
TONEGAWA, S
机构：
[1] UNIV CALIF LOS ANGELES,SCH MED,INST MOLEC BIOL,LOS ANGELES,CA 90024
[2] UNIV CALIF LOS ANGELES,SCH MED,DEPT MICROBIOL & IMMUNOL,LOS ANGELES,CA 90024
来源：
NATURE | 1979年 / 277卷 / 5698期
关键词：
D O I：
10.1038/277627a0
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A 6.8-kilobase DNA fragment containing the sequence coding for the constant region of the mouse immunoglobulin γ1 heavy chain was cloned from total cellular DNA. Electron microscopic and nucleotide sequencing studies showed that the three protein domains and the hinge region are encoded in separate DNA segments. © 1979 Nature Publishing Group.
引用
收藏
页码：627 / 633
页数：7
相关论文
共 44 条
[31]  
ROGERS JG, UNPUBLISHED
[32]   CLONED MPC 11 MYELOMA CELLS EXPRESS 2 KAPPA GENES - GENE FOR A COMPLETE LIGHT CHAIN AND A GENE FOR A CONSTANT REGION POLYPEPTIDE [J].
ROSE, SM ;
KUEHL, WM ;
SMITH, GP .
CELL, 1977, 12 (02) :453-462
[33]   USE OF THIN ACRYLAMIDE GELS FOR DNA SEQUENCING [J].
SANGER, F ;
COULSON, AR .
FEBS LETTERS, 1978, 87 (01) :107-110
[34]   ANTIBODY ACTIVE SITES AND IMMUNOGLOBULIN MOLECULES [J].
SINGER, SJ ;
DOOLITTL.RF .
SCIENCE, 1966, 153 (3731) :13-&
[35]   DETECTION OF SPECIFIC SEQUENCES AMONG DNA FRAGMENTS SEPARATED BY GEL-ELECTROPHORESIS [J].
SOUTHERN, EM .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (03) :503-+
[36]   IMPROVED DERIVATIVE OF A PHAGE LAMBDA EK2 VECTOR FOR CLONING RECOMBINANT DNA [J].
TIEMEIER, D ;
ENQUIST, L ;
LEDER, P .
NATURE, 1976, 263 (5577) :526-527
[37]   INTERVENING SEQUENCE OF DNA IDENTIFIED IN STRUCTURAL PORTION OF A MOUSE BETA-GLOBIN GENE [J].
TILGHMAN, SM ;
TIEMEIER, DC ;
SEIDMAN, JG ;
PETERLIN, BM ;
SULLIVAN, M ;
MAIZEL, JV ;
LEDER, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (02) :725-729
[38]   INTERVENING SEQUENCE OF A MOUSE BETA-GLOBIN GENE IS TRANSCRIBED WITHIN 15S BETA-GLOBIN MESSENGER-RNA PRECURSOR [J].
TILGHMAN, SM ;
CURTIS, PJ ;
TIEMEIER, DC ;
LEDER, P ;
WEISSMANN, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (03) :1309-1313
[39]   CLONING OF AN IMMUNOGLOBULIN VARIABLE REGION GENE FROM MOUSE EMBRYO [J].
TONEGAWA, S ;
BRACK, C ;
HOZUMI, N ;
SCHULLER, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (08) :3518-3522
[40]   STRUCTURE OF YEAST PHENYLALANINE-TRANSFER-RNA GENES - INTERVENING DNA SEGMENT WITHIN REGION CODING FOR TRANSFER-RNA [J].
VALENZUELA, P ;
VENEGAS, A ;
WEINBERG, F ;
BISHOP, R ;
RUTTER, WJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (01) :190-194
12345