PURIFICATION AND CHARACTERIZATION OF A CHYMOTRYPSIN KUNITZ INHIBITOR TYPE OF POLYPEPTIDE FROM THE VENOM OF COBRA (NAJA-NAJA-NAJA)

被引：38

作者：

SHAFQAT, J

ZAIDI, ZH

JORNVALL, H

机构：

[1] KAROLINSKA INST,DEPT CHEM 1,S-10401 STOCKHOLM 60,SWEDEN

[2] UNIV KARACHI,HEJ RES INST CHEM,KARACHI 75270,PAKISTAN

来源：

FEBS LETTERS | 1990年 / 275卷 / 1-2期

关键词：

Amino acid sequence; Cobra; HPLC-FPLC seperation; Kunitz-type chymotrypsin inhibitor; Snake venom; Weak contact site replacement;

D O I：

10.1016/0014-5793(90)81426-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A chymotrypsin Kunitz inhibitor type of polypeptide has been isolated from the venom of Naja naja naja by reverse phase HPLC and cation exchange FPLC. It is present in a considerably lower amount than that of the corresponding trypsin inhibitor. The primary structure, determined by sequence analysis of the whole molecule and its tryptic peptides, has 57 residues with an apparent molecular mass of 6.2 kDa. The main contact site with the protease (P1) has a Phe, showing the specificity of the inhibitor. Of residues considered functionally important in Kunitz-type inhibitors, Gly-36 is replaced by Ser in a segment of weak contacts with the protease. © 1990.

引用

页码：6 / 8

页数：3

共 14 条

[1] SNAKE-VENOM PROTEINASE-INHIBITORS .3. ISOLATION OF 5 POLYPEPTIDE INHIBITORS FROM VENOMS OF HEMACHATUS-HAEMACHATUS (RINGHALS COBRA) AND NAJA-NIVEA (CAPE COBRA) AND COMPLETE AMINO-ACID SEQUENCES OF 2 OF THEM [J].