ISOLATION, PURIFICATION AND CHARACTERIZATION OF NEW SITE-SPECIFIC ENDONUCLEASES BCIBI AND BCIBII PRODUCED BY BACILLUS-CIRCULANS

被引：0

作者：

SAMKO, OT ^{[1
]}

KALUGIN, AA ^{[1
]}

ELDAROV, MA ^{[1
]}

KARPYCHEV, IV ^{[1
]}

ANIKEITCHEVA, NV ^{[1
]}

KHOROSHUTINA, EB ^{[1
]}

SKRYABIN, KG ^{[1
]}

LIBRIK, GI ^{[1
]}

SOKOLOV, NN ^{[1
]}

机构：

[1] RUSSIAN ACAD SCI, VA ENGELHARDT INST MOLEC BIOL, CTR BIOENGN, MOSCOW, RUSSIA

来源：

BIOORGANICHESKAYA KHIMIYA | 1994年 / 20卷 / 12期

关键词：

RESTRICTION ENDONUCLEASES; SPECIFICITY;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

New site-specific endonucleases BciBI and BciBII have been detected in Bacillus circulans. The enzymes were purified by fractionation of cell-free extract with polyethylene imine and ammonium sulphate (40-80% of saturation) followed by chromatography on DEAE-sepharose, blue-sepharose and phosphocellulose. The endonucleases BciBI and BciBII were separated only at the final step of the purification - by chromatography on the phosphocellulose column. The yields of BciBI and BciBII were 600 and 10 000 U/g of cells. It was found that restriction endonucleases BciBI and BciBII are isoschizomers of ClaI and BstNI, respectively.

引用

页码：1327 / 1333

页数：7

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