IS PHOSPHORYLATION THE MAIN PHYSIOLOGICAL ACTION OF MYOSIN LIGHT-CHAIN KINASE

被引：3

作者：

EBASHI, S ^{[1
]}

KUWAYAMA, H ^{[1
]}

机构：

[1] OBIHIRO UNIV AGR & VET MED,CHEM LAB,OBIHIRO,HOKKAIDO,JAPAN

来源：

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 1994年 / 72卷 / 11期

关键词：

MYOSIN LIGHT CHAIN KINASE; PHOSPHORYLATION OF MYOSIN LIGHT CHAIN; LEIOTONIN; WORTMANNIN; BERYLLIUM SULFATE;

D O I：

10.1139/y94-198

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The 155-kDa component of bovine stomach, which exhibits a strong actomyosin (AM) activating activity and a relatively weak myosin light chain kinase (MLCK) activity, has a strong affinity for the actin filament and the actin-binding site is confined to an 80 amino acid residue on its N-terminal side. This affinity may play a crucial role in AM activation. Some reagents preferentially abolish either the AM-activating effect or MLCK activity. In conclusion, MLCK of the 155-kDa component does not play a fundamental role in activating the AM system as far as the in vitro system is concerned. The possible mechanism of AM activation by the component is discussed.

引用

页码：1377 / 1379

页数：3

共 13 条

[1]

BREMEL RD, 1977, BIOCH SMOOTH MUSCLE, P551

[2] EFFECTS OF ANTIBODIES TO MYOSIN LIGHT CHAIN KINASE ON CONTRACTILITY AND MYOSIN PHOSPHORYLATION IN CHEMICALLY PERMEABILIZED SMOOTH-MUSCLE [J].

DELANEROLLE, P ;

STRAUSS, JD ;

FELSEN, R ;

DOERMAN, GE ;

PAUL, RJ .

CIRCULATION RESEARCH, 1991, 68 (02) :457-465

[3] SIMPLE METHOD OF PREPARING ACTIN-FREE MYOSIN FROM SMOOTH-MUSCLE [J].

EBASHI, S .

JOURNAL OF BIOCHEMISTRY, 1976, 79 (01) :229-231

[4]

EBASHI S, 1993, BIOMED RES-TOKYO, V14, P117

[5] EXCITATION-CONTRACTION COUPLING AND THE MECHANISM OF MUSCLE-CONTRACTION [J].

EBASHI, S .

ANNUAL REVIEW OF PHYSIOLOGY, 1991, 53 :1-16

[6]

EBASHI S, 1988, CALCIUM SIGNAL CELL, P143

[7]

EBASHI S, 1993, ANN REPORT RES GROUP, P1

[8]

EBASHI S, 1977, EXCITATION CONTRACTI, P325

[9]

EBASHI S, 1977, BIOCH SMOOTH MUSCLE, P551

[10] ISOLATION OF CDNA FOR BOVINE STOMACH 155 KDA PROTEIN EXHIBITING MYOSIN LIGHT CHAIN KINASE-ACTIVITY [J].

KOBAYASHI, H ;

INOUE, A ;

MIKAWA, T ;

KUWAYAMA, H ;

HOTTA, Y ;

MASAKI, T ;

EBASHI, S .

JOURNAL OF BIOCHEMISTRY, 1992, 112 (06) :786-791

← 1 2 →