A GEL-MEDIATED FEEDING TECHNIQUE FOR PROTEIN CRYSTAL-GROWTH FROM HANGING DROPS

被引：11
作者：
BERNARD, Y
DEGOY, S
LEFAUCHEUX, F
ROBERT, MC
机构：
[1] UNIV PARIS 06,MINERAL CRISTALLOG LAB,F-75252 PARIS 05,FRANCE
[2] UNIV PARIS 07,MINERAL CRISTALLOG LAB,F-72522 PARIS 05,FRANCE
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 1994年 / 50卷
关键词：
D O I：
10.1107/S0907444994001290
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A procedure which allows an investigator to supply a crystal with fresh mother material without inducing significant growth defects is described. This technique requires that the crystal is grown in a gelled hanging or sitting drop. An example concerning a model macromolecule, hen egg-white lysozyme, is given. Extension of this procedure to other macromolecules is discussed.
引用
收藏
页码：504 / 507
页数：4
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