STABLE EXPRESSION OF THE GUS REPORTER GENE IN CHRYSANTHEMUM DEPENDS ON BINARY PLASMID T-DNA

被引：41

作者：

DEJONG, J ^{[1
]}

MERTENS, MMJ ^{[1
]}

RADEMAKER, W ^{[1
]}

机构：

[1] RES STN ORNAMENTAL PLANT GROWING,B-9990 MOLLO,BELGIUM

来源：

PLANT CELL REPORTS | 1994年 / 14卷 / 01期

关键词：

AGROBACTERIUM; TRANSFORMATION; T-DNA;

D O I：

10.1007/BF00233300

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Three chrysanthemum (Dendranthema grandiflora) cultivars were cocultivated with 2 Agrobacterium tumefaciens strains in combination with 4 pBIN19 derived binary plasmids, all carrying the Nosnptll selection gene and 35Sgus(intron) reporter gene. All binary plasmids transferred DNA to chrysanthemum explants but only pMOG410 gave good stable expression of GUS. This plasmid differs from the other plasmids in 2 aspects: 1) It carries a restored nptll gene and 2) the selection gene is positioned at the left border side of the reporter gene. Cocultivation with AGLO(pMOG410) yielded up to 13 GUS positive shoots per 100 explants. The presence of the gus and nptll gene in recovered shoots was confirmed by PCR and Southern blot analysis.

引用

页码：59 / 64

页数：6

共 24 条

[11] THE HYPERVIRULENCE OF AGROBACTERIUM-TUMEFACIENS A281 IS ENCODED IN A REGION OF PTIBO542 OUTSIDE OF TRANSFER DNA [J].