CLONING OF THE HUMAN TAURINE TRANSPORTER AND CHARACTERIZATION OF TAURINE UPTAKE IN THYROID-CELLS

被引：116
作者：
JHIANG, SM
FITHIAN, L
SMANIK, P
MCGILL, J
TONG, Q
MAZZAFERRI, EL
机构：
[1] Department of Internal Medicine, The Ohio State University, Columbus
来源：
FEBS LETTERS | 1993年 / 318卷 / 02期
关键词：
HUMAN; THYROID; TAURINE; TRANSPORTER; CDNA; SEQUENCE;
D O I：
10.1016/0014-5793(93)80008-I
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cDNA clone encoding a taurine transporter, designated HTAU, has been isolated from human thyroid. It contains an open reading frame encoding a protein of 619 amino acids with a calculated molecular weight of 69,675 Da. The predicted amino acid sequence of HTAU is highly homologous to those of dog kidney and rat brain. The HTAU mRNA was detectable in various human tissues examined. Transient expression of HTAU in COS-7 cells conferred a higher taurine uptake. The taurine uptake in FRTL-5 cells appears to be regulated by thyrotropin through cAMP. Finally, a higher taurine uptake may be associated with a higher proliferation rate in some cultured cell lines.
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收藏
页码：139 / 144
页数：6
相关论文
共 18 条
  • [1] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [2] CULTURE OF HORMONE-DEPENDENT FUNCTIONAL EPITHELIAL-CELLS FROM RAT THYROIDS
    AMBESIIMPIOMBATO, FS
    PARKS, LAM
    COON, HG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06): : 3455 - 3459
  • [3] USEFULNESS OF TAURINE IN CHRONIC CONGESTIVE-HEART-FAILURE AND ITS PROSPECTIVE APPLICATION
    AZUMA, J
    SAWAMURA, A
    AWATA, N
    [J]. JAPANESE CIRCULATION JOURNAL-ENGLISH EDITION, 1992, 56 (01): : 95 - 99
  • [4] GAULL GE, 1983, SULFUR AMINO ACIDS B, P297
  • [5] CLONING AND EXPRESSION OF A RAT-BRAIN GABA TRANSPORTER
    GUASTELLA, J
    NELSON, N
    NELSON, H
    CZYZYK, L
    KEYNAN, S
    MIEDEL, MC
    DAVIDSON, N
    LESTER, HA
    KANNER, BI
    [J]. SCIENCE, 1990, 249 (4974) : 1303 - 1306
  • [6] JONES DP, 1991, J AM SOC NEPHROL, V2, P1021
  • [7] TAURINE INHIBITS PROTEIN KINASE-C-CATALYZED PHOSPHORYLATION OF SPECIFIC PROTEINS IN A RAT CORTICAL P2 FRACTION
    LI, YP
    LOMBARDINI, JB
    [J]. JOURNAL OF NEUROCHEMISTRY, 1991, 56 (05) : 1747 - 1753
  • [8] TAURINE - RETINAL FUNCTION
    LOMBARDINI, JB
    [J]. BRAIN RESEARCH REVIEWS, 1991, 16 (02) : 151 - 169
  • [9] PRIMARY STRUCTURE AND FUNCTIONAL EXPRESSION OF A CHOLINE TRANSPORTER EXPRESSED IN THE RAT NERVOUS-SYSTEM
    MAYSER, W
    SCHLOSS, P
    BETZ, H
    [J]. FEBS LETTERS, 1992, 305 (01) : 31 - 36
  • [10] Nozaki M, 1989, Rinsho Shinkeigaku, V29, P713
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