ANALYSIS OF PERIPHERAL-BLOOD OF PREGNANT-WOMEN FOR THE PRESENCE OF FETAL Y-CHROMOSOME-SPECIFIC ZFY GENE DEOXYRIBONUCLEIC-ACID SEQUENCES

被引：45

作者：

KAO, SM

TANG, GC

HSIEH, TT

YOUNG, KC

WANG, HC

PAO, CC

机构：

[1] CHANG GUNG MED COLL,DEPT BIOCHEM,259 WENHWA RD,TAOYUAN,TAIWAN

[2] CHANG GUNG MEM HOSP,DEPT OBSTET & GYNECOL,TAIPEI,TAIWAN

[3] TAIPEI MUNICIPAL WOMEN & CHILDREN HOSP,TAIPEI,TAIWAN

[4] NATL CHENG KUNG UNIV,COLL MED,DEPT MED TECHNOL,TAINAN,TAIWAN

来源：

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY | 1992年 / 166卷 / 03期

关键词：

ZFY GENE; POLYMERASE CHAIN REACTION; PERIPHERAL MATERNAL BLOOD; FETAL SEX DETERMINATION;

D O I：

10.1016/0002-9378(92)91381-J

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Deoxyribonucleic acid sequences of human ZFY (zinc-finger-Y) gene, a Y-chromosome-specific gene and candidate for the testis-determining factor, has been identified by an in vitro enzymatic deoxyribonucleic acid amplification method in peripheral blood specimens of women pregnant with male fetuses. This technique permits detection of ZFY gene deoxyribonucleic acid sequences in as few as a single male cell among 1,000,000 female cells. Maternal blood results were confirmed by amplification of ZFY gene deoxyribonucleic acid sequences in chronic villus cells and by karyotyping in 33 pregnant women. There was no false-positive male result, and two of the three blood specimens with false-negative results were obtained from pregnant women at a very early gestational age. With properly designed guidelines, this deoxyribonucleic acid amplification method may be an alternative to determine the fetal sex for those pregnancies at risk for X-linked genetic disorders.

引用

页码：1013 / 1019

页数：7