EFFECT OF DESIPRAMINE ON A GLYCOPROTEIN SIALYLTRANSFERASE ACTIVITY IN C6 CULTURED GLIOMA-CELLS

被引:7
|
作者
BROQUET, P [1 ]
BAUBICHONCORTAY, H [1 ]
GEORGE, P [1 ]
PESCHARD, MJ [1 ]
LOUISOT, P [1 ]
机构
[1] FAC MED LYON SUD,BIOCHEM GEN & MED LAB,INSERM,U189,POB 12,F-69921 OULLINS,FRANCE
关键词
C6 glioma cells; Cultured cells; Desipramine; Sialyltransferase;
D O I
10.1111/j.1471-4159.1990.tb01885.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The tricyclic antidepressant desipramine, when added to culture medium, gave rise in C6 rat glioma cells to a decrease of the activity of the enzyme asialofetuin sialyltransferase. The inhibition was dose and time dependent and was observed in both multiplying cells and cells blocked with 2 mM thymidine or depletion of amino acids. This inhibition was rather specific to the sialyltransferase, as under the conditions where this enzyme was inhibited up to 70%, other enzymes such as dolichol phosphate mannose synthetase, glutamine synthetase, and glycerol phosphate dehydrogenase remained unaffected. This inhibition was not reversed after removal of desipramine from the medium and was not observed by direct addition of desipramine to the sialyltransferase incubation assay. Under the same conditions, W‐7 [N‐(6‐aminohexyl)‐5‐chloro‐1‐naphthalenesulfonamide], which is known to be a potent calmodulin antagonist and an inhibitor of calmodulin‐dependent kinases, gave the same concentration‐dependent inhibition profile of sialyltransferase as desipramine, whereas H‐7 [1‐(5‐isoquinolinylsulfonyl)‐2‐methylpiperazine], which is an inhibitor of protein kinase C and cyclic nucleotide‐dependent kinases, had no effect. So, it is suggested that desipramine inhibits the sialyltransferase activity in C6 glioma cells through a calmodulin‐dependent system. Copyright © 1990, Wiley Blackwell. All rights reserved
引用
收藏
页码:388 / 394
页数:7
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