RACH2, A NOVEL HUMAN GENE THAT COMPLEMENTS A FISSION YEAST-CELL CYCLE CHECKPOINT MUTATION

被引：16

作者：

DAVEY, S ^{[1
]}

BEACH, D ^{[1
]}

机构：

[1] COLD SPRING HARBOR LAB,HOWARD HUGHES MED INST,COLD SPRING HARBOR,NY 11724

来源：

MOLECULAR BIOLOGY OF THE CELL | 1995年 / 6卷 / 10期

关键词：

D O I：

10.1091/mbc.6.10.1411

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have identified a novel human gene by virtue of its ability to complement the rad1-1 checkpoint mutant of Schizosaccharomyces pombe. This gene, called RACH2, rescues the temperature-sensitive lethality of a rad1-1 wee1-50 double mutant of S. pombe. Expression of RACH2 in S. pombe rad1-1 strains partially restores UV resistance to the rad1-1 mutant strain. Expression of RACH2 in a rad1-1 cdc25-22 double mutant partially restores the dose-dependent delay in mitotic entry after irradiation that is lost in rad1-1 checkpoint-deficient mutants. Overexpression of RACH2 in human tissue culture cells induces apoptosis.

引用

页码：1411 / 1421

页数：11

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