MUSCARINE INCREASES A VOLTAGE-INDEPENDENT POTASSIUM CONDUCTANCE THROUGH AN M(4) RECEPTOR IN RAT DORSOLATERAL SEPTAL NUCLEUS NEURONS

被引：5

作者：

HASUO, H ^{[1
]}

AKASU, T ^{[1
]}

GALLAGHER, JP ^{[1
]}

机构：

[1] UNIV TEXAS,MED BRANCH,DEPT PHARMACOL & TOXICOL,GALVESTON,TX 77555

来源：

NEUROSCIENCE LETTERS | 1995年 / 189卷 / 03期

关键词：

SEPTUM; POTASSIUM CHANNEL; HYPERPOLARIZATION; MUSCARINIC RECEPTOR; PERTUSSIS TOXIN; G-PROTEIN;

D O I：

10.1016/0304-3940(95)11483-D

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The direct effect of muscarine on neurons of the rat dorsolateral septal nucleus (DLSN) was examined by using conventional microelectrode and voltage-clamp techniques. Muscarine (1-50 mu M) caused a hyperpolarization accompanied by an increase of a voltage-independent potassium conductance. Pirenzepine competitively antagonized the muscarine-induced hyperpolarization with an apparent dissociation constant (K-d) Value of 54 nM. Furthermore, intracellular loading with GTP gamma S, a non-hydrolyzable GTP analog, blocked irreversibly the muscarine-induced hyperpolarization. In addition, pretreatment of neurons with pertussis toxin (PTX) prevented the hyperpolarization produced by muscarine. These results suggest that muscarine hyperpolarizes DLSN neurons via a voltage-independent potassium conductance by acting at M(4) subtype receptors which are coupled to a PTX-sensitive G-protein in DLSN neurons.

引用

页码：163 / 166

页数：4

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