Nifedipine and tetrodotoxin delay the onset of methylmercury-induced increase in [Ca2+](i) in NG108-15 cells

被引:29
作者
Hare, MF
Atchison, WD
机构
[1] MICHIGAN STATE UNIV, DEPT PHARMACOL & TOXICOL, NEUROSCI PROGRAM, E LANSING, MI 48824 USA
[2] MICHIGAN STATE UNIV, INST ENVIRONM TOXICOL, E LANSING, MI 48824 USA
关键词
D O I
10.1006/taap.1995.1236
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Methylmercury (MeHg) causes a multiphasic disruption of intraneuronal cation regulation, Release of Ca2+ from internal stores and entry of extracellular Ca2+ (Ca-e(2+)) contribute to the temporally distinct early (first Ca2+ phase) and late (second Ca2+ phase) components of increased intracellular Ca2+ concentration ([Ca2+](i)). The present study was designed to explore the mechanisms mediating the second Ca2+ phase, Fluorescence intensity was monitored from single NG108-15 cells loaded with fura-2 before and during acute application of 2 mu M MeHg. Nifedipine (1 or 10 mu M but not 0.1 mu M) significantly delayed the time-to-onset of the second Ca2+ phase. Nifedipine (1 mu M but not 0.1 mu M) also caused a concentration-dependent delay in the onset of both the first Ca2+ phase which is independent of Ca-e(2+) and the elevation of non-Ca2+ cation (non-Ca2+ phase). The L-type dihydropyridine (DHP) Ca2+ channel agonist Bay K-8644 (10 nM) had no effect on the time-to-onset of the second Ca2+ phase. Neither the N-type Ca2+ channel blocker omega-conotoxin GVIA (up to 1 mu M) nor the nonselective Ca2+ channel blocker Ni2+ (1 mM) altered the time-to-onset of the second Ca2+ phase, Removal of Na-e(+) or addition of the voltage-dependent Na+ channel antagonist tetrodotoxin (TTX, 1 mu M) significantly delayed the onset of the second Ca2+ phase, In a manner similar to that for 1 mu M nifedipine, TTX also delayed the onset of the other phases, Thus, we hypothesize that MeHg depolarizes the plasma membrane leading to an increase in the activation of voltage-dependent Na+ and Ca2+ channels which promotes, directly or indirectly, the influx of Ca2+ during the second Ca2+ phase. (C) 1996 Academic Press, Inc.
引用
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页码:299 / 307
页数:9
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