PXEX, A VECTOR FOR EFFICIENT EXPRESSION OF CLONED SEQUENCES IN XENOPUS EMBRYOS

被引：77

作者：

JOHNSON, AD ^{[1
]}

KRIEG, PA ^{[1
]}

机构：

[1] UNIV TEXAS,CTR DEV BIOL,DEPT ZOOL,AUSTIN,TX 78712

来源：

GENE | 1994年 / 147卷 / 02期

关键词：

PLASMID; MICROINJECTION; EF-1-ALPHA PROMOTER; BLASTULA; TADPOLE; CAT REPORTER;

D O I：

10.1016/0378-1119(94)90070-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We describe the construction of pXeX, a plasmid vector that efficiently expresses cloned sequences in Xenopus embryos. This plasmid contains the transcription regulatory regions from the Xenopus laevis elongation factor-1 alpha-encoding gene (EF-1 alpha). Expression of cloned sequences commences in blastula-stage embryos, coincident with transcriptional activation of the embryonic genome, and transcripts may persist until the tadpole stage of development.

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页码：223 / 226

页数：4

相关论文

共 15 条

[1] DIFFERENTIAL EXPRESSION OF THE XENOPUS-LAEVIS TADPOLE AND ADULT BETA-GLOBIN GENES WHEN INJECTED INTO FERTILIZED XENOPUS-LAEVIS EGGS [J].

BENDIG, MM ;

WILLIAMS, JG .

MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (03) :567-570

[2] INTERACTIONS BETWEEN XWNT-8 AND SPEMANN ORGANIZER SIGNALING PATHWAYS GENERATE DORSOVENTRAL PATTERN IN THE EMBRYONIC MESODERM OF XENOPUS [J].

CHRISTIAN, JL ;

MOON, RT .

GENES & DEVELOPMENT, 1993, 7 (01) :13-28

[3] TRANSFORMED XENOPUS EMBRYOS AS A TRANSIENT EXPRESSION SYSTEM TO ANALYZE GENE-EXPRESSION AT THE MIDBLASTULA TRANSITION [J].

ETKIN, LD ;

BALCELLS, S .

DEVELOPMENTAL BIOLOGY, 1985, 108 (01) :173-178

[4] REPLICATION, INTEGRATION AND EXPRESSION OF EXOGENOUS DNA INJECTED INTO FERTILIZED-EGGS OF XENOPUS-LAEVIS [J].

ETKIN, LD ;

PEARMAN, B ;

ROBERTS, M ;

BEKTESH, SL .

DIFFERENTIATION, 1984, 26 (03) :194-202

[5] RECOMBINANT GENOMES WHICH EXPRESS CHLORAMPHENICOL ACETYLTRANSFERASE IN MAMMALIAN-CELLS [J].

GORMAN, CM ;

MOFFAT, LF ;

HOWARD, BH .

MOLECULAR AND CELLULAR BIOLOGY, 1982, 2 (09) :1044-1051

[6] USE OF THE HUMAN ELONGATION FACTOR-1-ALPHA PROMOTER AS A VERSATILE AND EFFICIENT EXPRESSION SYSTEM [J].

KIM, DW ;

UETSUKI, T ;

KAZIRO, Y ;

YAMAGUCHI, N ;

SUGANO, S .

GENE, 1990, 91 (02) :217-223

[7] DEVELOPMENTAL REGULATION OF A GASTRULA-SPECIFIC GENE INJECTED INTO FERTILIZED XENOPUS EGGS [J].

KRIEG, PA ;

MELTON, DA .

EMBO JOURNAL, 1985, 4 (13A) :3463-3471

[8] THE MESSENGER-RNA ENCODING ELONGATION-FACTOR 1-ALPHA (EF-1-ALPHA) IS A MAJOR TRANSCRIPT AT THE MIDBLASTULA TRANSITION IN XENOPUS [J].

KRIEG, PA ;

VARNUM, SM ;

WORMINGTON, WM ;

MELTON, DA .

DEVELOPMENTAL BIOLOGY, 1989, 133 (01) :93-100

[9] FUNCTIONAL MESSENGER-RNAS ARE PRODUCED BY SP6 INVITRO TRANSCRIPTION OF CLONED CDNAS [J].

KRIEG, PA ;

MELTON, DA .

NUCLEIC ACIDS RESEARCH, 1984, 12 (18) :7057-7070

[10] LACZ EXPRESSION IN GERMLINE TRANSGENIC ZEBRAFISH CAN BE DETECTED IN LIVING EMBRYOS [J].

LIN, S ;

YANG, S ;

HOPKINS, N .

DEVELOPMENTAL BIOLOGY, 1994, 161 (01) :77-83