STIMULATION OF ARACHIDONIC-ACID METABOLISM BY A STREPTOCOCCAL PREPARATION (OK-432) IN RAT PERITONEAL-MACROPHAGES

被引：1

作者：

WATANABE, M

SHISHIDO, Y

HIRASAWA, N

MUE, S

FUJIKI, H

OHUCHI, K

机构：

[1] TOHOKU UNIV,COLL MED SCI,SENDAI,MIYAGI 980,JAPAN

[2] NATL CANC CTR,RES INST,DIV CANC CHEMOPREVENT,TOKYO 104,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1138卷 / 02期

关键词：

OK-432; PROSTAGLANDIN-E2; ARACHIDONIC ACID; PHAGOCYTOSIS; CYTOCHALASIN-B; (RAT PERITONEAL MACROPHAGE);

D O I：

10.1016/0925-4439(92)90056-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A streptococcal preparation OK-432 is reported to be an immunopotentiator and a potent antitumor agent. In order to elucidate the mechanism of biologic action, effects of OK-432 on arachidonic acid metabolism in rat peritoneal macrophages were investigated. Prostaglandin E2 production and release of radioactivity from [H-3]arachidonic acid-labeled macrophages were found to be stimulated by OK-432 in a concentration-dependent manner (5 to 80-mu-g/ml). Heat-treatment of OK-432 further stimulated its effects. These stimulative effects on arachidonic acid metabolism by OK-432 were not observed in MDCK cells that have no phagocytotic activity. Furthermore, cytochalasin B treatment completely suppressed the stimulative effects induced by OK-432 in macrophages. These results strongly indicate that the stimulative effects by OK-432 on arachidonic acid metabolism are dependent on phagocytosis of OK-432 particles. Significance of stimulation of arachidonic acid metabolism in macrophages by OK-432 for its biological effects is discussed.

引用

页码：157 / 161

页数：5

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