THE IMMUNOSUPPRESSIVE AGENT-FK506 INHIBITS INVITRO EXPRESSION OF MEMBRANE-BOUND AND SOLUBLE INTERLEUKIN-2 RECEPTORS ON RESTING BUT NOT ON ACTIVATED HUMAN-LYMPHOCYTES

被引:6
作者
KARLSSON, H [1 ]
TRUEDSSON, L [1 ]
NASSBERGER, L [1 ]
机构
[1] UNIV LUND,DEPT MED MICROBIOL,CLIN IMMUNOL SECT,SOLVEGATAN 23,S-22362 LUND,SWEDEN
关键词
FK506; INTERLEUKIN-2; RECEPTOR; HUMAN LYMPHOCYTE; FLOW CYTOMETRY; ELISA;
D O I
10.1016/0165-2478(91)90101-F
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
FK506 is a recently introduced immunosuppressive agent synthesised by the microorganism Streptomyces tskubaensis. It has been found to be more potent than Cyclosporin A in inhibiting T cell activation. We investigated its effects on the expression of membrane bound as well as soluble interleukin-2 receptors on human lymphocytes. The membrane-bound IL-2 receptor expression was inhibited by FK506 in resting lymphocytes at a concentration of 1 pmol/l. At 10 nmol/l no further inhibition was seen. In activated lymphocytes FK506 exerted no inhibitory effect on the IL-2 receptor expression. The release of soluble IL-2 receptor showed a pronounced decline in the concentration interval between 10 pmol/l and 0.1 nmol/l. Above a concentration of 10 nmol/l, no further decrease was seen. In activated lymphocytes the expression of soluble IL-2 receptors was unaffected by FK506 incubated up to 72 h. Pretreatment of the lymphocytes with the compound did not further depress the expression of the membrane-bound or the solube receptor. Our results also indicate that the expression of the membrane-bound receptor is more sensitive to the drug than the soluble form of the receptor.
引用
收藏
页码:129 / 132
页数:4
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