OBSERVATION OF LARGE SUBUNIT PROTEIN COMPLEXES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：78
作者：
LOO, JA
机构：
[1] Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan, 48105
来源：
JOURNAL OF MASS SPECTROMETRY | 1995年 / 30卷 / 01期
关键词：
D O I：
10.1002/jms.1190300127
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Mass spectrometry with electrospray ionization and a magnetic sector instrument was used to detect multiply charged molecules for the non-covalently bound dimeric subunit protein complexes of horse liver alcohol dehydrogenase (M(r) similar to 80 000) and the tetrameric complexes of yeast alcohol dehydrogenase (M(r) similar to 147 000) and rabbit muscle pyruvate kinase (M(r) similar to 232 000). Ions for the pyruvate kinase complex represent one of the largest intact protein complexes resolved by mass spectrometry. Solvation of the large gas phase complexes is indicated by the mass spectrometric results.
引用
收藏
页码：180 / 183
页数：4
相关论文
共 46 条
[11]   ELECTROSPRAY IONIZATION FOR MASS-SPECTROMETRY OF LARGE BIOMOLECULES [J].
FENN, JB ;
MANN, M ;
MENG, CK ;
WONG, SF ;
WHITEHOUSE, CM .
SCIENCE, 1989, 246 (4926) :64-71
[12]   PROBES OF SUBUNIT ASSEMBLY AND RECONSTITUTION PATHWAYS IN MULTISUBUNIT PROTEINS [J].
FRIEDMAN, FK ;
BEYCHOK, S .
ANNUAL REVIEW OF BIOCHEMISTRY, 1979, 48 :217-250
[13]   OBSERVATION OF NONCOVALENT ENZYME SUBSTRATE AND ENZYME PRODUCT COMPLEXES BY ION-SPRAY MASS-SPECTROMETRY [J].
GANEM, B ;
LI, YT ;
HENION, JD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (20) :7818-7819
[14]   DETECTION OF NONCOVALENT RECEPTOR LIGAND COMPLEXES BY MASS-SPECTROMETRY [J].
GANEM, B ;
LI, YT ;
HENION, JD .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (16) :6294-6296
[15]   MASS-SPECTROMETRIC DETECTION OF THE NONCOVALENT GDP-BOUND CONFORMATIONAL STATE OF THE HUMAN H-RAS PROTEIN [J].
GANGULY, AK ;
PRAMANIK, BN ;
TSARBOPOULOS, A ;
COVEY, TR ;
HUANG, E ;
FUHRMAN, SA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (16) :6559-6560
[16]   DIRECT OBSERVATION OF A DNA QUADRUPLEX BY ELECTROSPRAY IONIZATION MASS-SPECTROMETRY [J].
GOODLETT, DR ;
CAMP, DG ;
HARDIN, CC ;
CORREGAN, M ;
SMITH, RD .
BIOLOGICAL MASS SPECTROMETRY, 1993, 22 (03) :181-183
[17]   A STUDY OF THE THERMAL-DENATURATION OF RIBONUCLEASE-S BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY [J].
GOODLETT, DR ;
LOO, RRO ;
LOO, JA ;
WAHL, JH ;
UDSETH, HR ;
SMITH, RD .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1994, 5 (07) :614-622
[18]  
HILLENKAMP F, 1991, ANAL CHEM, V63, pA1193
[19]   INVESTIGATION OF SYMMETRY OF OLIGOMERIC ENZYMES WITH BIFUNCTIONAL REAGENTS [J].
HUCHO, F ;
MULLNER, H ;
SUND, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1975, 59 (01) :79-87
[20]   AN APPROACH TO THE ELUCIDATION OF THE QUATERNARY STRUCTURE ROLE IN THE ACTIVITY OF PYRUVATE-KINASE STUDIES ON THE IMMOBILIZED ENZYME [J].
JEDRZEJAK, J ;
HEYDUK, T ;
KOCHMAN, M .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY, 1983, 15 (05) :695-702
12345