OBSERVATION OF LARGE SUBUNIT PROTEIN COMPLEXES BY ELECTROSPRAY-IONIZATION MASS-SPECTROMETRY

被引：78
作者：
LOO, JA
机构：
[1] Parke-Davis Pharmaceutical Research, Division of Warner-Lambert Company, Ann Arbor, Michigan, 48105
来源：
JOURNAL OF MASS SPECTROMETRY | 1995年 / 30卷 / 01期
关键词：
D O I：
10.1002/jms.1190300127
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Mass spectrometry with electrospray ionization and a magnetic sector instrument was used to detect multiply charged molecules for the non-covalently bound dimeric subunit protein complexes of horse liver alcohol dehydrogenase (M(r) similar to 80 000) and the tetrameric complexes of yeast alcohol dehydrogenase (M(r) similar to 147 000) and rabbit muscle pyruvate kinase (M(r) similar to 232 000). Ions for the pyruvate kinase complex represent one of the largest intact protein complexes resolved by mass spectrometry. Solvation of the large gas phase complexes is indicated by the mass spectrometric results.
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页码：180 / 183
页数：4
相关论文
共 46 条
[1]   DIRECT OBSERVATION OF A TERNARY COMPLEX BETWEEN THE DIMERIC ENZYME HIV-1 PROTEASE AND A SUBSTRATE-BASED INHIBITOR [J].
BACA, M ;
KENT, SBH .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (10) :3992-3993
[2]  
Branden C-I., 1975, ENZYMES, V11, P103, DOI 10.1016/S1874-6047(08)60211-5
[3]   YEAST ALCOHOL DEHYDROGENASE - SH GROUPS, DISULFIDE GROUPS, QUATERNARY STRUCTURE, AND REACTIVATION BY REDUCTIVE CLEAVAGE OF DISULFIDE GROUPS [J].
BUHNER, M ;
SUND, H .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1969, 11 (01) :73-&
[4]  
COTTAM GL, 1969, J BIOL CHEM, V244, P1481
[5]  
DAVIES GE, 1962, CAN J BIOCHEM, V50, P416
[6]   NEW PROTEIN REAGENTS - N-(4-CHLOROMERCURIPHENYL)-4-CHLORO-3,5-DINITROBENZAMIDE AND ITS USE AS A PROBE OF QUATERNARY STRUCTURE OF YEAST ALCOHOL-DEHYDROGENASE [J].
DIOPOH, J ;
OLOMUCKI, M .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1977, 75 (02) :441-444
[7]   REVERSIBLE SOLVENT DENATURATION OF RABBIT MUSCLE PYRUVATE-KINASE [J].
DOSTER, W ;
HESS, B .
BIOCHEMISTRY, 1981, 20 (04) :772-780
[8]   ASSESSING THE MULTIMERIC STATES OF PROTEINS - STUDIES USING LASER DESORPTION MASS-SPECTROMETRY [J].
FARMER, TB ;
CAPRIOLI, RM .
BIOLOGICAL MASS SPECTROMETRY, 1991, 20 (12) :796-800
[9]  
FARMER TB, 1993, TECHNIQUES PROTEIN C, V4, P73
[10]   ANALYSIS OF ANTIBODIES AND OTHER LARGE GLYCOPROTEINS IN THE MASS RANGE OF 150000-200000 DA BY ELECTROSPRAY IONIZATION MASS-SPECTROMETRY [J].
FENG, R ;
KONISHI, Y .
ANALYTICAL CHEMISTRY, 1992, 64 (18) :2090-2095
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