POSTEMBEDDING STAINING OF BRAIN-TISSUE FOR ULTRASTRUCTURAL VISUALIZATION OF AMYLOID IN ALZHEIMERS-DISEASE

被引：1

作者：

MIGHELI, A

ATTANASIO, A

GIORDANA, MT

机构：

[1] Neurological Clinic II, University of Torino, Torino

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1993年 / 68卷 / 02期

关键词：

AMYLOID; METHENAMINE SILVER STAIN; LR GOLD RESIN; IMMUNOGOLD; ELECTRON MICROSCOPY; ALZHEIMERS DISEASE;

D O I：

10.3109/10520299309104678

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A postembedding staining method is presented for ultrastructural visualization of amyloid deposits in brain sections from patients with Alzheimer's disease. Methenamine silver stain is applied to thin sections of tissue embedded in the acrylic resin LR Gold. Senile plaques are easily labeled by silver granules and the ultrastructural detail is well preserved. When staining time is prolonged, silver precipitate also is deposited on neuronal paired helical filaments. This method overcomes the drawbacks of previously reported applications of the stain on Vibratome and Epon sections. Thin sections from the same tissue block can be immunostained with antibodies to various plaque components, thus allowing comparative studies at the electron microscope level.

引用

页码：117 / 121

页数：5

共 9 条

[1]

ATTANASIO A, 1991, 18 P C IT SOC EL MIC, P35

[2]

BEHROUZ N, 1989, LAB INVEST, V61, P576

[3] AN ENHANCED METHOD FOR POSTEMBEDDING IMMUNOCYTOCHEMICAL STAINING WHICH PRESERVES CELL-MEMBRANES [J].