ELECTROPHYSIOLOGICAL AND PHARMACOLOGICAL PROPERTIES OF SINGLE SPINAL NEURONS ISOLATED FROM ADULT BULLFROGS

被引：2

作者：

ADACHI, S ^{[1
]}

OKA, JI ^{[1
]}

FUKUDA, H ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,DEPT PHARMACOL & TOXICOL,BUNKYO KU,TOKYO 113,JAPAN

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY | 1990年 / 95卷 / 02期

关键词：

D O I：

10.1016/0742-8413(90)90114-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. We developed an isolated spinal cell preparation from adult bullfrogs. 2. 2. The average resting membrane potential was - 60mV, and an action potential was activated by positive current injection. 3. 3. The cells retained their tetrodotoxin-sensitive Na+ channels and at least two kinetically different types of K+ channel. 4. 4. Under K+-free conditions, responses to GABA were blocked by bicuculline while responses to glycine, taurine or β-alanine were blocked by strychnine. 5. 5. The potency of excitatory amino acids decreased in the order: kainic acid > glutamate > NMDA. 6. 6. These studies demonstrated that the isolated cells are applicable for electrophysiological and pharmacological investigations. © 1990.

引用

页码：253 / 263

页数：11

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