ANTIBODY TO B-SUBTILIS DNA POLYMERASE-III - USE IN ENZYME-PURIFICATION AND EXAMINATION OF HOMOLOGY AMONG REPLICATION-SPECIFIC DNA-POLYMERASES

被引：26

作者：

BARNES, MH

BROWN, NC

机构：

[1] Department of Pharmacology, University of Massachusetts Medical School, Worcester

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 03期

关键词：

D O I：

10.1093/nar/6.3.1203

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus subtilis DNA polymerase III (pol III), an arylhydrazinopyrimidine-sensitive, replication-specific enzyme, was used to generate a non-precipitating rabbit antibody which specifically inhibited pol III activity in vitro. The antibody was used to examine structural relationships among several DNA polymerases, and it was linked covalently to agarose; the antibody:agarose was employed to develop a rapid, selective method of purification of catalytically active B.subtilis pol III. © 1979 Information Retrieval Limited.

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页码：1203 / 1219

页数：17

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