RNA DUPLEX UNWINDING ACTIVITY OF ALFALFA MOSAIC-VIRUS RNA-DEPENDENT RNA-POLYMERASE

被引：9
作者：
DEGRAAFF, M
HOUWING, CJ
LUKACS, N
JASPARS, EMJ
机构：
[1] Institute of Molecular Plant Sciences, Plant Virology Section, Gorlaeus Laboratories, NL-2300 RA Leiden
来源：
FEBS LETTERS | 1995年 / 371卷 / 03期
关键词：
ALFALFA MOSAIC VIRUS; RNA-DEPENDENT RNA POLYMERASE; RNA DUPLEX UNWINDING;
D O I：
10.1016/0014-5793(95)00875-A
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An RNA-dependent RNA polymerase (RdRp) purified from alfalfa mosaic virus-infected tobacco is capable of synthesizing in vitro full-size RNAs of minus and plus polarities. However, the enzyme is not able to perform a complete replication cycle in vitro. The products were found to be completely base-paired to their templates. The enzyme was able to use double-stranded RNA as a template for RNA synthesis if it could initiate from a single-stranded promoter, The inability (of most) of our enzyme preparations to create a single-stranded initiation site could explain why they could not perform a complete replication cycle in vitro, This is the first report on duplex RNA unwinding activities by a plant viral RdRp.
引用
收藏
页码：219 / 222
页数：4
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