STRUCTURAL CHARACTERIZATION OF PNA-DNA DUPLEXES BY NMR - EVIDENCE FOR DNA IN A B-LIKE CONFORMATION

被引：94
作者：
LEIJON, M
GRASLUND, A
NIELSEN, PE
BUCHARDT, O
NORDEN, B
KRISTENSEN, SM
ERIKSSON, M
机构：
[1] UNIV COPENHAGEN,PANUM INST,IMBG,CTR BIOMOLEC RECOGNIT,LAB B,DK-2200 COPENHAGEN N,DENMARK
[2] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT BIOPHYS,S-10691 STOCKHOLM,SWEDEN
[3] UNIV COPENHAGEN,HC ORSTED INST,DEPT CHEM,DK-2100 COPENHAGEN 0,DENMARK
[4] CHALMERS UNIV TECHNOL,DEPT PHYS CHEM,S-41296 GOTHENBURG,SWEDEN
来源：
BIOCHEMISTRY | 1994年 / 33卷 / 33期
关键词：
D O I：
10.1021/bi00199a002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by H-1 NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.
引用
收藏
页码：9820 / 9825
页数：6
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