STRUCTURAL CHARACTERIZATION OF PNA-DNA DUPLEXES BY NMR - EVIDENCE FOR DNA IN A B-LIKE CONFORMATION

被引：94

作者：

LEIJON, M

GRASLUND, A

NIELSEN, PE

BUCHARDT, O

NORDEN, B

KRISTENSEN, SM

ERIKSSON, M

机构：

[1] UNIV COPENHAGEN,PANUM INST,IMBG,CTR BIOMOLEC RECOGNIT,LAB B,DK-2200 COPENHAGEN N,DENMARK

[2] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT BIOPHYS,S-10691 STOCKHOLM,SWEDEN

[3] UNIV COPENHAGEN,HC ORSTED INST,DEPT CHEM,DK-2100 COPENHAGEN 0,DENMARK

[4] CHALMERS UNIV TECHNOL,DEPT PHYS CHEM,S-41296 GOTHENBURG,SWEDEN

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 33期

关键词：

D O I：

10.1021/bi00199a002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by H-1 NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.

引用

收藏

页码：9820 / 9825

页数：6

相关论文

共 50 条

[31] TOXI 58-Structural characterization of DNA duplexes having exocyclic 2'-deoxyguanosine adducts [J].

de los Santos, Carlos ;

Zaliznyak, Tanya ;

Bonala, Radha R. ;

Johnson, Francis ;

Lukin, Mark .

ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2006, 232

[32] An asymmetric NFAT1 dimer on a pseudo-palindromic κB-like DNA site [J].

Jin, L ;

Sliz, P ;

Chen, L ;

Macián, F ;

Rao, A ;

Hogan, PG ;

Harrison, SC .

NATURE STRUCTURAL BIOLOGY, 2003, 10 (10) :807-811

[33] CHARACTERIZATION OF THE STRUCTURAL CONFORMATION ADOPTED BY (TTAGGG)(N) TELOMERIC DNA REPEATS OF DIFFERENT LENGTH IN CLOSED CIRCULAR DNA [J].

HUERTAS, D ;

LIPPS, H ;

AZORIN, F .

JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 1994, 12 (01) :79-90

[34] Enzymic characterization of a novel member of the regulatory B-like carboxypeptidase with transcriptional repression function: stimulation of enzymic activity by its target DNA [J].

Muise, AM ;

Ro, HS .

BIOCHEMICAL JOURNAL, 1999, 343 :341-345

[35] Enzymic characterization of a novel member of the regulatory B-like carboxypeptidase with transcriptional repression function: Stimulation of enzymic activity by its target DNA [J].

Muise, Aleixo M. ;

Ro, Hyo-Sung .

Biochemical Journal, 1999, 343 (pt 2) :341-345

[36] NMR structural and kinetic characterization of a homeodomain diffusing and hopping on nonspecific DNA [J].

Iwahara, Junji ;

Zweckstetter, Markus ;

Clore, G. Marius .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (41) :15062-15067

[37] Sequence and organization of genomic DNA of two factor B-like genes in the nurse shark (Ginglymotoma cirratum) [J].

Shin, Dong-Ho ;

Smith, Sylvia L. .

MOLECULAR IMMUNOLOGY, 2007, 44 (1-3) :239-239

[38] Cationic liposomes as delivery systems for double-stranded PNA-DNA chimeras exhibiting decoy activity against NF-κB transcription factors [J].

Borgatti, M ;

Breda, L ;

Cortesi, R ;

Nastruzzi, C ;

Romanelli, A ;

Saviano, M ;

Bianchi, N ;

Mischiati, C ;

Pedone, C ;

Gambari, R .

BIOCHEMICAL PHARMACOLOGY, 2002, 64 (04) :609-616

[39] Structural insights into the effect of isonucleosides on B-DNA duplexes using molecular-dynamics simulations [J].

Hongwei Jin ;

Suxin Zheng ;

Zhanli Wang ;

Cheng Luo ;

Jianhua Shen ;

Hualiang Jiang ;

Liangren Zhang ;

Lihe Zhang .

Journal of Molecular Modeling, 2006, 12 :781-791

[40] Structural insights into the effect of isonucleosides on B-DNA duplexes using molecular-dynamics simulations [J].

Jin, Hongwei ;

Zheng, Suxin ;

Wang, Zhanli ;

Luo, Cheng ;

Shen, Jianhua ;

Jiang, Hualiang ;

Zhang, Liangren ;

Zhang, Lihe .

JOURNAL OF MOLECULAR MODELING, 2006, 12 (06) :781-791

← 1 2 3 4 5 →