STRUCTURAL CHARACTERIZATION OF PNA-DNA DUPLEXES BY NMR - EVIDENCE FOR DNA IN A B-LIKE CONFORMATION

被引：94

作者：

LEIJON, M

GRASLUND, A

NIELSEN, PE

BUCHARDT, O

NORDEN, B

KRISTENSEN, SM

ERIKSSON, M

机构：

[1] UNIV COPENHAGEN,PANUM INST,IMBG,CTR BIOMOLEC RECOGNIT,LAB B,DK-2200 COPENHAGEN N,DENMARK

[2] UNIV STOCKHOLM,ARRHENIUS LAB,DEPT BIOPHYS,S-10691 STOCKHOLM,SWEDEN

[3] UNIV COPENHAGEN,HC ORSTED INST,DEPT CHEM,DK-2100 COPENHAGEN 0,DENMARK

[4] CHALMERS UNIV TECHNOL,DEPT PHYS CHEM,S-41296 GOTHENBURG,SWEDEN

来源：

BIOCHEMISTRY | 1994年 / 33卷 / 33期

关键词：

D O I：

10.1021/bi00199a002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The nucleic acid analogues PNA (peptide nucleic acids) hybridize with DNA of complementary sequence. The solution structures of two PNA-DNA duplexes, H-(GCTATGTC)-NH2.d(GACATAGC) and H-(GTAGATCACT)-NH2.d(AGTGATCTAC), have been studied by H-1 NMR. It was found that the PNA-DNA hybrids are base paired by hydrogen bonds, most likely of the Watson-Crick type. From two-dimensional NOESY and COSY results it is concluded that the DNA strand in the PNA-DNA complex adopts a B-like structure with the deoxyribose sugars in the C2'-endo conformation.

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收藏

页码：9820 / 9825

页数：6

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