CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF PROTOCATECHUATE 3,4-DIOXYGENASE FROM ACINETOBACTER-CALCOACETICUS

被引：27

作者：

VETTING, MW ^{[1
]}

EARHART, CA ^{[1
]}

OHLENDORF, DH ^{[1
]}

机构：

[1] UNIV MINNESOTA,DEPT BIOCHEM,KAHLERT STRUCT BIOL LAB,MINNEAPOLIS,MN 55455

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 236卷 / 01期

关键词：

PROTOCATECHUATE 3,4-DIOXYGENASE; METALLOENZYMES; ACINETOBACTER CALCOACETICUS; X-RAY DIFFRACTION; CRYSTALLIZATION;

D O I：

10.1006/jmbi.1994.1142

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 Å. The dodecahedral crystals diffract to beyond 2.5 Å resolution. The asymmetric unit contains one twelfth of the enzyme (αβFe+3)12 complex. © 1994 Academic Press Limited.

引用

页码：372 / 373

页数：2