CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF PROTOCATECHUATE 3,4-DIOXYGENASE FROM ACINETOBACTER-CALCOACETICUS

被引：27
作者：
VETTING, MW [1 ]
EARHART, CA [1 ]
OHLENDORF, DH [1 ]
机构：
[1] UNIV MINNESOTA,DEPT BIOCHEM,KAHLERT STRUCT BIOL LAB,MINNEAPOLIS,MN 55455
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1994年 / 236卷 / 01期
关键词：
PROTOCATECHUATE 3,4-DIOXYGENASE; METALLOENZYMES; ACINETOBACTER CALCOACETICUS; X-RAY DIFFRACTION; CRYSTALLIZATION;
D O I：
10.1006/jmbi.1994.1142
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
X-ray quality single crystals of protocatechuate 3,4-dioxygenase from Acinetobacter calcoaceticus were obtained by the hanging drop method. The intradiol dioxygenase crystallizes in the cubic space group I23 with unit cell dimensions a = b = c = 145.5 Å. The dodecahedral crystals diffract to beyond 2.5 Å resolution. The asymmetric unit contains one twelfth of the enzyme (αβFe+3)12 complex. © 1994 Academic Press Limited.
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页码：372 / 373
页数：2
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