3-DIMENSIONAL WORKING MODEL OF M1 RNA, THE CATALYTIC RNA SUBUNIT OF RIBONUCLEASE-P FROM ESCHERICHIA-COLI

被引：90

作者：

WESTHOF, E ^{[1
]}

ALTMAN, S ^{[1
]}

机构：

[1] YALE UNIV, DEPT BIOL, NEW HAVEN, CT 06520 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 11期

关键词：

PRECURSOR TRANSFER-RNA; COMPUTER MODELING;

D O I：

10.1073/pnas.91.11.5133

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A three-dimensional model of M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli, was constructed with the aid of a computer. The modeling process took into account data from chemical and enzymatic protection experiments, phylogenetic analysis, studies of the activities of mutants, and the kinetics of reactions catalyzed by the binding of substrate to M1 RNA. The model provides a plausible picture of the binding to M1 RNA of the tRNA domain of a precursor tRNA substrate. The scissile bond and adjacent segments of the aminoacyl acceptor stem of a precursor tRNA substrate can fit into a cleft that leads to the phylogenetically conserved, central part of the structure.

引用

页码：5133 / 5137

页数：5

共 42 条

[21] SUBSTRATE RECOGNITION BY RNASE-P AND BY THE CATALYTIC M1 RNA - IDENTIFICATION OF POSSIBLE CONTACT POINTS IN PRE-TRANSFER RNAS [J].