SPECIFIC CHEMICAL LABELING OF DNA FRAGMENTS

被引：31
作者：
ESHAGHPOUR, H
SOLL, D
CROTHERS, DM
机构：
[1] YALE UNIV,DEPT MOLEC BIOPHYS,NEW HAVEN,CT 06520
[2] YALE UNIV,DEPT BIOCHEM,NEW HAVEN,CT 06520
来源：
NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 06期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1093/nar/7.6.1485
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a simple method for specific chemical labeling of DNA fragments at their 3'-termin1. The procedure includes enzymatic addition of 4-th1ourid1ne, followed by reaction in mild non-denaturing conditions with the highly reactive α-haloacetamido derivatives of several chemical labels. The attached reporter molecule can be removed by extended treatment with β-mercaptoethanol. Among the potential applications of this labeling method is the study of specific protein-DNA interactions in solution. © 1979 Information Retrieval Limited.
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页码：1485 / 1495
页数：11
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