ISOLATION AND SEQUENCE-ANALYSIS OF THE CHLAMYDIA-PNEUMONIAE GROE OPERON

被引：38
作者：
KIKUTA, LC [1 ]
PUOLAKKAINEN, M [1 ]
KUO, CC [1 ]
CAMPBELL, LA [1 ]
机构：
[1] UNIV WASHINGTON,DEPT PATHOBIOL,SEATTLE,WA 98195
来源：
INFECTION AND IMMUNITY | 1991年 / 59卷 / 12期
关键词：
D O I：
10.1128/IAI.59.12.4665-4669.1991
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Chlamydia pneumoniae has emerged as an important human respiratory pathogen. From a lambda gt11 gene bank constructed from C. pneumoniae isolate AR-39 DNA, an immunoreactive plaque containing a 3.0-kb insert was purified. In immunoblots, a 60-kDa protein was recognized by anti-C. pneumoniae rabbit immune serum. The recombinant protein was reactive with a Chlamydia genus-specific monoclonal antibody recognizing a 60-kDa protein found in the Sarkosyl-soluble fraction and with rabbit immune serum prepared against the Chlamydia trachomatis 60-kDa GroEL homolog associated with the delayed-type hypersensitivity response. DNA sequence analysis confirmed that the C. pneumoniae gene product is an analog of the C. trachomatis delayed-type hypersensitivity antigen and the Escherichia coli GroEL heat shock protein.
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页码：4665 / 4669
页数：5
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