A RAPID PREPARATION METHOD FOR ULTRASTRUCTURAL INVESTIGATIONS OF CONIFER NEEDLES

被引：2
作者：
EBEL, B
HAMELMANN, U
NIESS, C
机构：
[1] Ruhr-Universität, Bochum, D-4630
来源：
JOURNAL OF MICROSCOPY-OXFORD | 1990年 / 160卷
关键词：
Conifer needle; preparation method; ultrastructure;
D O I：
10.1111/j.1365-2818.1990.tb03048.x
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
The method discussed here for preparing conifer needles for transmission electron microscopy combines three important components in a new way: chilling (273 K) during fixation to prevent autolysis, HEPES buffer to protect cytoplasm against washing out and VCD/HXSA as an ultralow viscid and therefore rapidly infiltrating medium. Ultrastructural analysis of Picea abies and Pinus silvestris shows very satisfactory tissue preservation. 1990 Blackwell Science Ltd
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页码：67 / 74
页数：8
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