DOPAMINE-RECEPTORS EXPRESSED IN THE XENOPUS-OOCYTES INJECTED WITH BOVINE STRIATAL MESSENGER-RNA

被引：8
作者：
SAKAI, Y
SEKIGUCHI, M
OKAMOTO, K
KUWANO, RZ
TAKAHASHI, Y
机构：
[1] NATL DEF MED COLL,DEPT PHARMACOL,3-2 NAMIKI,TOKOROZAWA,SAITAMA 359,JAPAN
[2] NIIGATA UNIV,BRAIN RES INST,DEPT NEUROPHARMACOL,NIIGATA 95021,JAPAN
来源：
MOLECULAR BRAIN RESEARCH | 1990年 / 7卷 / 02期
关键词：
Desensitization; Dopamine receptor; mRNA; Striatum; Xenopus oocyte;
D O I：
10.1016/0169-328X(90)90097-W
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The response to dopamine (DA) of Xenopus oocytes injected with bovine striatal mRNA was electrophysiologically and pharmacologically investigated under a voltage-clamped condition. In oocytes injected with bovine striatal mRNA and then treated with collagenase (denuded oocytes), DA applied by superfusion dose-dependently induced oscillatory inward currents with a long latency and an ED50 value of 12.0 μM. mRNA non-injected denuded oocytes did not respond to DA up to concentrations of 1 mM. The reversal potential of DA-induced currents was about -25 mV, indicating that DA increased a Cl--conductance. DA-induced currents were easily desensitized by repeated applications of DA. Haloperidol and SCH-23390 abolished DA-induced currents, while propranolol and mianserin showed no effect. Furthermore, SK&F-38393, a specific agonist of the DA D1 receptor, induced similar oscillatory currents as DA did. These results suggest the possibility that functional D1 and D2 DA receptors were co-expressed in Xenopus oocytes by injection of bovine striatal mRNA. © 1990.
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收藏
页码：183 / 187
页数：5
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